FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Dys, Dg
FlyBase ID
FBig0000102656
Interaction Network
Interactions Browser links
Dys network
Dg network
Reported Interactions
FBrf0191923-1.FPS
Reference
Yatsenko et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dys 

dystrophin

bait

His tag

Dys 
Dg 

dystroglycan

prey

tetramethylrhodamine label

Dg 
Dg 
Dg 
Dg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dys 
WW-EF hand region
sufficient binding region
aa 2470-2739

coordinates relative to Dys-PC

Dg 
WW domain binding site I
sufficient binding region
GKSPATPYRKPPPYVSP

Kd = 16uM

Dg 
WW domain binding site II
sufficient binding region
PVILKDEKPPLLPPSYNT

Kd = 46uM

Dg 
WW domain binding site I
mutation decreasing interaction
GKSPATPYRKWPPYVSP

Kd = 178uM

Dg 
WW domain binding site II
mutation decreasing interaction
PVILKDEKPPLLPPSGNT

Kd = 147uM

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized and labeled in vitro.

(Yatsenko et al., 2007)
FBrf0192541-1.FPS
Reference
Shcherbata et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dys 

Dys

bait

His tag

Dg 

Dg

prey

fluorescein label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized and labeled in vitro.

(Shcherbata et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)