Source was cell extract of HEK 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

Given that ppk26 can bind ppk and that both colocalize to the same dendritic regions suggests that they may form a hetermultimeric channel in vivo.

ppk and ppk26 show mutually dependent surface expression: mutation of one prevents cell surface localization of the other.

This interaction is detected only in nociceptive class IV multidentritic neurons, and not in class I-III neurons that also coexpress both transgenic constructs.

Source was live larvae of transgenic fly line; proteins produced from tagged transgenic constructs.