FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mwh, cmb
FlyBase ID
FBig0000103140
Interaction Network
Interactions Browser links
mwh network
cmb network
Reported Interactions
FBrf0226208-3.Y2H
Reference
Fagan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mwh 

Mwh

bait

fused to GAL4 DNA-binding domain

cmb 

Cmb

prey

fused to GAL4 activation domain

cmb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cmb 
N-terminal region
sufficient binding region
aa 1-856

coordinates relative to cmb-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fagan et al., 2014)

cmb interacts with mwh, but not with in, fy or frtz.

(Fagan et al., 2014)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Fagan et al., 2014)
FBrf0226208-4.coIP.WB
Reference
Fagan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cmb 

Cmb

bait

GFP tag

cmb 
mwh 

Mwh

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cmb 
N-terminal region
sufficient binding region
aa 279-856

coordinates relative to cmb-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fagan et al., 2014)

Both the long cmb-PA and short cmb-PB isoforms interact with mwh in this assay.

(Fagan et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)