FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103402
Interaction Network
Interactions Browser links
ens network
Khc network
Reported Interactions
FBrf0218014-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ens 

Ens

bait

fused to GAL4 DNA-binding domain

Khc 

Khc

prey

fused to GAL4 activation domain

Khc 
Khc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Khc 
motor and stalk domain
sufficient binding region
aa 239-573
Khc 
stalk domain
sufficient binding region
aa 408-827
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0241995-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ens 

Ensc

bait

GFP tag

ens 
Khc 

KHC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ens 
KBD domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was female adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)