FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(spl)m3-HLH, ac
FlyBase ID
FBig0000103570
Interaction Network
Interactions Browser links
E(spl)m3-HLH network
ac network
Reported Interactions
FBrf0099085-9.Y2H
Reference
Alifragis et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(spl)m3-HLH 

m3

bait

fused to LexA DNA-binding domain

E(spl)m3-HLH 
ac 

Ac

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(spl)m3-HLH 
bHLH domain
sufficient binding region
aa 1-77
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Alifragis et al., 1997)

E(spl)m3-HLH interacts with sc, ac, ase and gro, but not with da or run, in this assay.

(Alifragis et al., 1997)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Alifragis et al., 1997)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)