FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103705
Interaction Network
Interactions Browser links
Nep4 network
CG3534 network
Reported Interactions
FBrf0219388-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nep4 

Nep4

bait

fused to GAL4 DNA-binding domain

Nep4 

CG3534

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nep4 
intracellular domain
sufficient binding region
aa 1-56

coordinates relative to Nep4-PA isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from normalized universal library).

Two-hybrid system: yeast GAL4-BD/VP16-AD

FBrf0219388-2.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nep4 

Nep4

bait

His tag

Nep4 

CG3534

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nep4 
intracellular domain
sufficient binding region
aa 1-56

coordinates relative to Nep4-PA isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from baculovirus-infected Sf21 cell extract.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)