Interaction in vitro; inhibitor produced as a recombinant fusion protein in bacterial system; enzyme target produced by in vitro transcription.
Sxl inhibits translation of a luciferase reporter fused to the hoe1 5\' UTR.
The hoe1 5\' UTR was fused to a luciferase reporter and translated using a Drosophila embryo translation extract in the presence or absence of recombinant Sxl. Translation was determined by measuring luciferase activity in the reaction after translation.
