Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from extract of transgenic adult male testis.
The Rpb4 binding site in the Cycb 3 prime UTR is required for translational repression of a reporter construct in early spermatocytes.
Biotin labeled CycB RNA pulled down using streptavidin beads. Bound Rpb4 protein detected by anti-GFP western blot.
