FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pk
FlyBase ID
FBig0000103766
Interaction Network
Interactions Browser links
pk network
Reported Interactions
FBrf0162094-4.PD.A
Reference
Jenny et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

GST tag

pk 

Pk

prey

[35]S label

pk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pk 
C-terminal region
sufficient binding region
aa 778-963

coordinates relative to pk-PA, construct "ΔN1"

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Jenny et al., 2003)
FBrf0225692-1.coIP.WB
Reference
Ayukawa et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

FLAG tag

pk 

Sple

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ayukawa et al., 2014)

The two distinct isoforms of the pk gene ("Pk" and "Sple") interact with each other, and also show homophilic interactions.

(Ayukawa et al., 2014)
FBrf0255277-4.coIP.WB
Reference
Chen et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

HA tag

pk 

Sple

prey

myc tag

pk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pk 
C terminal region
necessary binding region
aa unspecified

Deleted from the common exon fragment aa 14-963.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction occurs between the Pk and Sple isoforms of pk.

(Chen et al., 2023)

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (3)