Two-hybrid system: yeast GAL4-BD/GAL4-AD
Multiple regions of cnn can self-interact.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was embryos of transgenic fly line; bait produced from transgenic gene; prey produced from endogenous gene.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
The LZ domain forms a tetramer in solution.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
The CM2 domain forms a dimer in solution.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
A mixture of LZ and CM2 forms a 2:2 hetero-tetrameric complex.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
The LZ and CM2 domains of cnn form a 2:2 hetero-tetrameric complex. In the absence of LZ domain, CM2 domain homodimers can interact in anti-parallel to form a tetramer in crystallo.
PReM domain includes the LZ domain and additional N- and C-terminal extensions.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
Unlike the smaller LZ domain, the PReM domain forms a homodimer in solution rather than a tetramer, while a mixture of PReM and CM2 forms much larger complex than the LZ-CM2 hetero-tetrameric complex.
coordinates relative to cnn-PA
coordinates relative to cnn-PA
Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.
The estimated molecular weights are consistent with dimers.
L1131A,L1137A, coordinates relative to cnn-PA
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
