Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced by in vitro translation.

Interaction shown for the cytoplasmic isoform of how, how(S).

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The long form of how specifically binds Nrx-IV RNA (not Grasp65 RNA), while the short form of how specifically binds Grasp65 RNA (not Nrx-IV RNA).