FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
how, Grasp65
FlyBase ID
FBig0000103859
Interaction Network
Interactions Browser links
how network
Grasp65 network
Reported Interactions
FBrf0224073-1.coIP.PCR
Reference
Giuliani et al., 2014
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
how 

HOW

bait
Grasp65 

dgrasp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Grasp65 HRE1 is sufficient to mediate RNA stability but not targeting of Grasp65 mRNA to the open ZOC ("zone of contact") in the follicular epithelium.

(Giuliani et al., 2014)

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Giuliani et al., 2014)
FBrf0224073-3.coIP.WB
Reference
Giuliani et al., 2014
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
how 

HOW

bait

HA tag

how 
Grasp65 

dgrasp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Giuliani et al., 2014)

The short form of how specifically binds Grasp65 RNA (not Nrx-IV RNA), while the long form of how specifically binds Nrx-IV RNA (not Grasp65 RNA).

(Giuliani et al., 2014)
FBrf0224073-5.coIP.MW
Reference
Giuliani et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
how 

HOW

bait

HA tag

how 
Grasp65 

dgrasp

prey
Grasp65 
Grasp65 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Grasp65 
interaction domain
sufficient binding region
nt 801-1383

"CT2" fragment, coordinates relative to Grasp65-RA CDS

Grasp65 
HRE1 motif
necessary binding region
aa 872-881

CCTAAC, "CT2 ΔHRE1" fragment, coordinates relative to Grasp65-RA CDS

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro translation; prey produced by in vitro transcription.

(Giuliani et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)