Lid
FLAG tag
H3
H3K4me3 or H3K4me2
Interaction in vitro; enzyme produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey peptide chemically synthesized.
His3
biotinylated
lid
GST
Methylation at K4 eliminates binding
Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.
Interaction shown with PHD1 domain of lid only and N-terminal 21 aa of His3, not full-length proteins.
Requires di- or tri-methylation at K4.
Interaction shown with PHD3 domain of lid only and N-terminal 21 aa of His3, not full-length proteins.
