H3
biotin tag, acetylated at K9 and K14
FSH
His tag
Interaction in vitro; bait chemically synthesized; prey produced as a recombinant fusion protein in bacterial system.
fs(1)h binds acetylated His3 peptides more strongly than unmodified peptides. Binding of fs(1)h to His3 is also inhibited by the BET bromodomain inhibitor JQ1.