H4
biotin tag, acetylated at K5, K8, K12 and K16
FSH
His tag
Interaction in vitro; bait chemically synthesized; prey produced as a recombinant fusion protein in bacterial system.
fs(1)h binds acetylated His4 peptides more strongly than unmodified peptides. Binding of fs(1)h to His4 is also inhibited by the BET bromodomain inhibitor JQ1.