FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
pk, dgo
FlyBase ID
FBig0000104150
Interaction Network
Interactions Browser links
pk network
dgo network
Reported Interactions
FBrf0180143-1.PD.A
Reference
Das et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

GST tag

pk 
dgo 

Dgo

prey

[35]S label

dgo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pk 
C-terminus region
necessary binding region
aa 822-909

aa coordinates inferred from citation FBrf0162094

dgo 
ankryin repeats
sufficient binding region
aa 1-263

aa coordinates inferred from primer sequences.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Das et al., 2004)
FBrf0213849-4.PD.A
Reference
Mirkovic et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

GST tag

pk 
dgo 

Dgo

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pk 
PkC
sufficient binding region

This assay uses "the part of Pk common to all isoforms" but makes no further specifications. Further investigation into the citations also yields no specification beyond that these constructs encapsulate binding domains common to all isoforms of pk that are found in the last two exons of the gene.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Mirkovic et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)