FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
trx, Snr1
FlyBase ID
FBig0000104266
Interaction Network
Interactions Browser links
trx network
Snr1 network
Reported Interactions
FBrf0155964-5.Y2H
Reference
Marenda et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trx 

TRX

bait

fused to GAL4 DBD

trx 
Snr1 

SNR1

prey

fused to GAL4 activation domain

Snr1 
Snr1 
Snr1 
Snr1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Snr1 
interaction domain
sufficient binding region
aa 44-261

coordinates relative to Snr1-PA

Snr1 
interaction domain
necessary binding region
aa 239-260

coordinates relative to Snr1-PA

Snr1 
interaction domain residue 256
mutation decreasing interaction
aa 256

G256D, coordinates relative to Snr1-PA; G256D has reduced interaction at 20C and decreasd interaction at 30C. Effect of G256D mutation studied in the context of aa 44-370 of Snr1.

Snr1 
interaction domain residue 249
mutation disrupting interaction
aa 249

V249A, coordinates relative to Snr1-PA; V294A loses all interaction. Effect of V249A mutation studied in the context of aa 44-370 of Snr1.

trx 
SET domain
sufficient binding region
aa 3534-3759
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent.

(Marenda et al., 2003)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Marenda et al., 2003)
FBrf0224162-8.PD.WB
Reference
Tie et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trx 

TRX

bait

GST tag

trx 
trx 
Snr1 

SNR1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trx 
SET domain
sufficient binding region
aa 3476-3726

coordinates relative to trx-PA

trx 
SET domain
necessary binding region
aa 3574-3726

coordinates relative to trx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic nuclear extract.

(Tie et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)