FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104397
Interaction Network
Interactions Browser links
ft network
Reported Interactions
FBrf0208364-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

fat

bait

FLAG tag

ft 

fat

prey

HA tag

ft 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ft 
interaction domain
sufficient binding region
aa 1-301
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

Two forms of ft were used in this experiment. A ft construct omiting the extracellular region and another which includes the first 53 amino acids of Fat fused to a fragment from amino acid 4429 to the stop codon were used in this experiment.

FBrf0208364-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

Fat

bait

HA tag

ft 

Fat

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

A single ft transgenic construct with a MYC tag at the N terminus and a HA tag at the C terminus was used in this experiment.

FBrf0208364-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ft 

Fat

bait

MYC tag

ft 

Fat

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

A single transgenic construct with a MYC tag at the N terminus and a HA tag at the C terminus was used in this experiment.

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References (1)