Source was larval eye discs; bait produced from transgenic construct; prey produced from endogenous gene.

Overexpression of mir-7 reduces aop protein levels.

A GFP reporter fused to the 3\'UTR of aop was downregulated in photoreceptor cells. Mutation of the four predicted mir-7 binding sites in the aop 3\'UTR abrogated this downregulation.

GFP reporter assay

Mutation of mir-7 leads to upregulation of aop protein.

Source was larval lymph glands of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Overexpression of mir-7 leads to downregulation of aop protein.

Source was larval lymph glands of mutant fly line; bait produced from transgenic construct; prey produced from endogenous gene.