Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Purified Spc105R was mixed with either the Kmn1-Mis12-Nnf1a or Kmn1-Mis12-Nnf1b trimer, and interaction was assessed by gel filtration and analytical centrifugation.

Spc105R can bind either the Kmn1-Mis12-Nnf1a or Kmn1-Mis12-Nnf1b trimer to form a complex that is 93.3 kDa or 95.7 kDa in size, respectively.