FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Spc105R, Kmn1
FlyBase ID
FBig0000104609
Interaction Network
Interactions Browser links
Spc105R network
Kmn1 network
Reported Interactions
FBrf0208678-2.Y2H
Reference
Schittenhelm et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kmn1 

Nsl1

bait

fused to GAL4 DNA-binding domain

Spc105R 

Spc105

prey

fused to GAL4 activation domain

Spc105R 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Spc105R 
C-terminal domain
sufficient binding region
aa 1330-1959

coordinates relative to Spc105R-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Schittenhelm et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Schittenhelm et al., 2009)
FBrf0208678-3.Y2H
Reference
Schittenhelm et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spc105R 

Spc105

bait

fused to GAL4 DNA-binding domain

Spc105R 
Kmn1 

Nsl1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Spc105R 
C-terminal domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Schittenhelm et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Schittenhelm et al., 2009)
FBrf0231098-24.CH.MW
Reference
Liu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spc105R 

Spc105R

neutral component
Spc105R 

Spc105R

neutral component
Spc105R 
Spc105R 

Spc105R

neutral component
Spc105R 
Kmn1 

Kmn1

neutral component

Hpc tag

Kmn1 

Kmn1

neutral component

Hpc tag

Kmn1 

Kmn1

neutral component

Hpc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Spc105R 
C-terminal region
sufficient binding region
aa 1707-1960
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified Spc105R was mixed with either the Kmn1-Mis12-Nnf1a or Kmn1-Mis12-Nnf1b trimer, and interaction was assessed by gel filtration and analytical centrifugation.

(Liu et al., 2016)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Liu et al., 2016)

Spc105R can bind either the Kmn1-Mis12-Nnf1a or Kmn1-Mis12-Nnf1b trimer to form a complex that is 93.3 kDa or 95.7 kDa in size, respectively.

(Liu et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)