FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ey, dac
FlyBase ID
FBig0000104615
Interaction Network
Interactions Browser links
ey network
dac network
Reported Interactions
FBrf0217470-1.coIP.WB
Reference
Okamoto et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ey 

ey

bait

GFP tag

dac 

dac

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Okamoto et al., 2012)
FBrf0217470-2.coIP.WB
Reference
Okamoto et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dac 

dac

bait

GFP tag

dac 
dac 
ey 

ey

prey

FLAG tag

ey 
ey 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ey 
paired domain
sufficient binding region
aa 1-460

coordinates relative to ey-PD

ey 
paired domain
necessary binding region
aa 1-232

coordinates relative to ey-PD

dac 
N-terminal domain
sufficient binding region
aa 1-358

coordinates relative to dac-PD

dac 
N-terminal domain
necessary binding region
aa 1-358

coordinates relative to dac-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Okamoto et al., 2012)
FBrf0217470-4.PD.WB
Reference
Okamoto et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ey 

ey

bait

GST tag

ey 
dac 

dac

prey

GFP tag

dac 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ey 
paired domain
sufficient binding region
aa 1-351

coordinates relative to ey-PD

dac 
DD1 domain
sufficient binding region
aa 180-358

coordinates relative to dac-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct in HEK293T cells.

(Okamoto et al., 2012)
FBrf0230121-3.coIP.WB
Reference
Okamoto and Nishimura, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ey 

Ey

bait

FLAG tag

dac 

Dac

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction interrupted by co-expression of a constitutively active form of foxo.

(Okamoto and Nishimura, 2015)

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Okamoto and Nishimura, 2015)
FBrf0230121-4.coIP.WB
Reference
Okamoto and Nishimura, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dac 

Dac

bait

FLAG tag

ey 

Ey

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Okamoto and Nishimura, 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)