Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system.

Components of the "WRC" complex were co-expressed in Sf9 cells (HSPC300, Sra-1, Abi, SCAR, Hem), pulled down via GST tag, and further purified by size exclusion column.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system.

Components of the "WRC" complex were co-expressed in Sf9 cells (HSPC300, Sra-1, Abi, SCAR, Hem), pulled down via MBP tag, and further purified by size exclusion column.

Purified WAVE regulatory complex (WRC).

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

Pull down was carried out with all proteins WRC complex (Hem, SCAR, Abi, Cyfip, and HSPC300). WRC complex captured as four pairwise interactions with HSPC300 as bait.