FLAG-HA tandem affinity purification.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Interaction of mio and Nprl3 is increased in the starved state in a wild type background. In an Wdr59 mutant background, the interaction is increased in both the fed and starved states compared to wild type and an Wdr24 mutant background.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.