FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pim, thr
FlyBase ID
FBig0000104893
Interaction Network
Interactions Browser links
pim network
thr network
Reported Interactions
FBrf0139669-1.Y2H
Reference
Jager et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thr 

THR

bait

fused to GAL4 DNA-binding domain

thr 
thr 
pim 

PIM

prey

fused to GAL4 activation domain

pim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
thr 
N-terminal region
sufficient binding region
aa 1-476
thr 
N-terminal region
necessary binding region
aa 1-476
pim 
C-terminal region
sufficient binding region
aa 115-199
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Jager et al., 2001)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Jager et al., 2001)
FBrf0139669-9.coIP.WB
Reference
Jager et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thr 

thr

bait

Myc tag

pim 

PIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Jager et al., 2001)
FBrf0160729-4.coIP.WB
Reference
Leismann and Lehner, 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pim 

PIM

bait

Myc tag

thr 

THR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Leismann and Lehner, 2003)

There were no differences in interaction between extracts produced from a transgenic fly line expressing wild-type pim or pim with a mutated D and Ken box.

(Leismann and Lehner, 2003)
FBrf0167373-1.Y2H
Reference
Jager et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pim 

PIM

bait

fused to GAL4 DNA-binding domain

pim 
pim 
pim 
thr 

THR

prey

fused to GAL4 activation domain

thr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
thr 
interaction domain
sufficient binding region
aa 1-933
pim 
interaction domain
necessary binding region
aa 142-199
pim 
interaction domain residue 2
mutation decreasing interaction
aa 2

unspecified aa substitution

pim 
interaction domain residue 187
mutation decreasing interaction
aa 187

unspecified aa substitution

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Jager et al., 2004)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Jager et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)