Interaction in vitro; protein produced in a baculovirus and insect cell system.
Crystal structure of hexameric complex captured as 15 pairwise interactions.
Embryonic nuclear extract was treated with DNase and RNase.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Embryonic nuclear extract was treated with DNase and RNase.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and High5 cell system.
The ORC complex visualized by EM was captured as 15 pairwise interactions. The addition of ATP caused the complex to adopt an active/open conformation.
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and High5 cell system.
Comparison of EM structures of ORC complex, ORC complex bound to DNA and a ternary complex consisting of ORC, DNA and Cdc6 curated as a series on binary protein-protein interactions.
Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and insect cell system.
