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FB2026_01
,
released March 12, 2026
Embryonic nuclear extract was treated with DNase and RNase.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Crude nuclear extract was subjected to several conventional chromatographic steps and gel filtration to purify the 700 kDa TREX-2 complex. This purified fraction was then used for immunoprecipitation.
The interaction was not affected by treatment with DNase or RNase.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system.