Crude nuclear extract was subjected to several conventional chromatographic steps and gel filtration to purify the 700 kDa TREX-2 complex. This purified fraction was then used for immunoprecipitation.
The interaction was not affected by treatment with DNase or RNase.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.