Source was embryos; bait produced from endogenous gene; prey produced from endogenous gene.
The snf-U1 snRNA interaction was curated to the products of all 5 identical or nearly identical U1 snRNA genes.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA cproduced by in vitro transcription.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro transcription.
Source was adult female ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.