FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000105247
Interaction Network
Interactions Browser links
aPKC network
orb2 network
Reported Interactions
FBrf0211209-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

DaPKC

bait

biotin label

aPKC 
orb2 

Orb2

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aPKC 
3-prime UTR
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type or transgenic adult head extract.

FBrf0225059-1.coIP.PCR
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
orb2 

Orb2

bait
aPKC 

apkc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males testes; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0225059-3.EMSA.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
orb2 

Orb2

bait
orb2 
aPKC 

apkc

prey

[32]P label

aPKC 
aPKC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
orb2 
RRM domain
sufficient binding region
aPKC 
3-prime UTR
sufficient binding region
aPKC 
CPE sequences
necessary binding region
UUUUAAU
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)