FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Zfrp8, RpS2
FlyBase ID
FBig0000105526
Interaction Network
Interactions Browser links
Zfrp8 network
RpS2 network
Reported Interactions
FBrf0233001-1.Y2H
Reference
Minakhina et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zfrp8 

Zrfp8

bait

fused to GAL4 DNA-binding domain

RpS2 

RpS2

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from a normalized universal library).

(Minakhina et al., 2016)

Five independent clones of RpS2 isolated in this screen.

(Minakhina et al., 2016)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Minakhina et al., 2016)
FBrf0233001-2.coIP.WB
Reference
Minakhina et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zfrp8 

Zfrp8

bait

TAP tag

RpS2 

RpS2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Minakhina et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)