FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mei-9, Ercc1
FlyBase ID
FBig0000105572
Interaction Network
Interactions Browser links
mei-9 network
Ercc1 network
Reported Interactions
FBrf0128640-1.Y2H
Reference
Sekelsky et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-9 

MEI-9

bait

fused to GAL4 DNA-binding domain

mei-9 
Ercc1 

ERCC1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-9 
C-terminal region
sufficient binding region
aa 252-926

coordinates relative to mei-9-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sekelsky et al., 2000)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Sekelsky et al., 2000)
FBrf0155944-2.Y2H
Reference
Yildiz et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-9 

MEI-9

bait

fused to GAL4 DNA-binding domain

Ercc1 

Ercc1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Yildiz et al., 2002)

Four of 26 positive clones in the mei-9 screen corresponded to Ercc1.

(Yildiz et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Yildiz et al., 2002)
FBrf0179099-2.Y2H
Reference
Yildiz et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-9 

MEI-9

bait

fused to GAL4 DNA-binding domain

mei-9 
mei-9 
Ercc1 

ERCC1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mei-9 
ERCC1 binding region
sufficient binding region
aa 600-926

coordinates relative to mei-9-PA

mei-9 
ERCC1 binding region
necessary binding region
aa 627-926

coordinates relative to mei-9-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Yildiz et al., 2004)

The interaction domains for Ercc1 and mus312 map to different regions of mei-9.

(Yildiz et al., 2004)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Yildiz et al., 2004)
FBrf0187655-1.Y2H
Reference
Radford et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ercc1 

ERCC1

bait

fused to GAL4 DNA-binding domain

mei-9 

MEI-9

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Radford et al., 2005)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Radford et al., 2005)
FBrf0207288-3.Y2H
Reference
Joyce et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ercc1 

ERCC1

bait

fused to GAL4 DNA-binding domain

mei-9 

MEI-9

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Joyce et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Joyce et al., 2009)

Positive control.

(Joyce et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)