Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

asf1 does not interact with Su(H), gro, or Caf1-55 in this experiment.

Caf1-55 was used as control in this experiment and did not interact with H, Su(H), gro or itself.

Two-hybrid system: yeast LexA-BD/B42-AD; positive control. Weak interaction with double incubation time compared to positive control.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

Myc-trap binding assay. Mapping demonstrated aa 315-338 are necessary for H interaction with asf1, but not sufficient on its own to support binding.