FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
aPKC, Myo10A
FlyBase ID
FBig0000105690
Interaction Network
Interactions Browser links
aPKC network
Myo10A network
Reported Interactions
FBrf0200619-30.PD.A
Reference
Liu et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait

GST tag

Myo10A 

Syph

prey

[35]S label

Myo10A 
Myo10A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myo10A 
FERM domain lobe 1
sufficient binding region
VPSVEEVTAVSAGRSARRQIYRLPGGAERVVNTRCSTVVADVIAELCALLGVESEAEQQEFSLYCIVQGDAFTMPLAADEYILDVTTELLKSGQPFYLIFCRSVWHFALKREPAP

construct "L1"

Myo10A 
FERM domain lobe 3
sufficient binding region
NVLATWPLFGSSFFAVKRIWAEEGPHVEDNHSPMWRDLILALNRRGVLFLDPNTHETLQHWSFMEVISTRKVRSEDGALFLDMKVGNLMQQRVIRVQ

construct "L3"

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Liu et al., 2008)
FBrf0200619-6.Y2H
Reference
Liu et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myo10A 

Syph

bait

fused to LexA DNA-binding domain

Myo10A 
aPKC 

aPKC

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myo10A 
tail region
sufficient binding region
aa 1838-3145

coordinates relative to Myo10A-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Liu et al., 2008)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Liu et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)