FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rlip, CycB
FlyBase ID
FBig0000105838
Interaction Network
Interactions Browser links
Rlip network
CycB network
Reported Interactions
FBrf0173158-1.Y2H
Reference
Rosse et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rlip 

Rlip

bait

fused to LexA DNA-binding domain

Rlip 
CycB 

cyclinB

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rlip 
C-terminal region
sufficient binding region
aa 482-625

coordinates relative to Rlip-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Rosse et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Rosse et al., 2003)

The Rlip two hybrid screen recovered 11 CycB clones.

(Rosse et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)