FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
EcR, Chd64
FlyBase ID
FBig0000106232
Interaction Network
Interactions Browser links
EcR network
Chd64 network
Reported Interactions
FBrf0202965-10.Y2H
Reference
Li et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait

fused to LexA DNA-binding domain

Chd64 

Chd64

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Li et al., 2007)

Two-hybrid system: yeast LexA-BD/B42-AD

(Li et al., 2007)
FBrf0202965-22.PD.A
Reference
Li et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait

GST tag

EcR 
Chd64 

Chd64

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EcR 
N terminal AB region deleted
sufficient binding region
aa 224-878

coordinates relative to EcR-PB, EcR-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Li et al., 2007)
FBrf0202965-6.Y2H
Reference
Li et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chd64 

Chd64

bait

fused to LexA DNA-binding domain

EcR 

EcR

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Li et al., 2007)

Two-hybrid system: yeast LexA-BD/B42-AD

(Li et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)