FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Dif, MED17
FlyBase ID
FBig0000106372
Interaction Network
Interactions Browser links
Dif network
MED17 network
Reported Interactions
FBrf0155963-21.PD.A
Reference
Park et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dif 

Dif

bait

GST tag

Dif 
MED17 

dTRAP80

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dif 
activation domain
sufficient binding region
aa 373-667

coordinates relative to Dif-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Park et al., 2003)
FBrf0155963-5.PD.WB
Reference
Park et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dif 

Dif

bait

GST tag

Dif 
MED17 

dTRAP80

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dif 
activation domain
sufficient binding region
aa 373-667

coordinates relative to Dif-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Park et al., 2003)
FBrf0180625-6.PD.A
Reference
Kim et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dif 

DIF

bait

GST tag

Dif 
MED17 

MED17

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dif 
activation domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kim et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)