Interaction in vitro; bait produced and labeled in vitro; prey produced as a recombinant fusion protein in bacterial system.

rhi binds only the methylated His3 H3K9me3 peptide, not the unmethylated form.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

rhi binds His3 H3K9me3 peptide with Kd = 21.8 uM, and an H3K9me2 peptide with Kd = 28.4 uM, but not H3K9me1 or unmethylated peptide.

rhi-His3 H3K9me3 peptide complex.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.