FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Skel, Asciz
FlyBase ID
FBig0000106817
Interaction Network
Interactions Browser links
Skel network
Asciz network
Reported Interactions
FBrf0234065-1.Y2H
Reference
Sengupta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Skel 

Skeletor

bait

fused to GAL4 DNA-binding domain

Skel 
Asciz 

Digitor

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Skel 
interaction domain
sufficient binding region
aa 183-442

coordinates relative to Skeletor-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Sengupta et al., 2016)

Two independent two hybrid screens using Skeletor as bait recovered Asciz.

(Sengupta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Sengupta et al., 2016)
FBrf0234065-2.PD.WB
Reference
Sengupta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Asciz 

Digitor

bait

GST tag

Asciz 
Skel 

Skeletor

prey

His tag

Skel 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Skel 
interaction domain
sufficient binding region
aa 183-442

coordinates relative to Skeletor-PD

Asciz 
C-terminal region
sufficient binding region
aa 144-376

coordinates relative to Asciz-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sengupta et al., 2016)
FBrf0234065-3.PD.WB
Reference
Sengupta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Skel 

Skeletor

bait

His tag

Skel 
Asciz 

Digitor

prey

GST tag

Asciz 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Skel 
interaction domain
sufficient binding region
aa 183-442

coordinates relative to Skeletor-PD

Asciz 
C-terminal region
sufficient binding region
aa 144-376

coordinates relative to Asciz-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sengupta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)