FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mad
FlyBase ID
FBig0000106933
Interaction Network
Interactions Browser links
Mad network
Reported Interactions
FBrf0104455-5.coIP.WB
Reference
Inoue et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

FLAG tag

Mad 

Mad

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS cell line; bait produced from transfected construct; prey produced from transfected construct.

(Inoue et al., 1998)
FBrf0201969-1.coIP.WB
Reference
Gao and Laughon, 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

FLAG tag

Mad 

Mad

prey

HA tag

Mad 
Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
MH2 domain
sufficient binding region
Mad 
linker domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gao and Laughon, 2007)
FBrf0201969-2.2H
Reference
Gao and Laughon, 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

fused to GAL4 DNA-binding domain

Mad 

Mad

prey

fused to VP16 activation domain

Mad 
Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
MH2 domain
sufficient binding region
Mad 
linker domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK293T cells; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Gao and Laughon, 2007)

Two-hybrid system: Mammalian GAL4-BD/VP16-AD

(Gao and Laughon, 2007)
FBrf0208242-1.CH.MW
Reference
Wang et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

MAD

self
Mad 

MAD

self
Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
MH2 domain phosphosites S453 and S455
mutation increasing interaction
aa 453-455

S453D + S455D (pseudophosphorylated), coordinates relative to Mad-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Wang et al., 2009)
FBrf0208242-2.XC
Reference
Wang et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

MAD

self
Mad 

MAD

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Wang et al., 2009)
External Crossreferences and Linkouts ( 0 )
References (3)