FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dl, tamo
FlyBase ID
FBig0000107002
Interaction Network
Interactions Browser links
dl network
tamo network
Reported Interactions
FBrf0158946-15.coIP.WB
Reference
Minakhina et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dl 

Dorsal

bait
tamo 

Tamo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Minakhina et al., 2003)
FBrf0158946-3.Y2H
Reference
Minakhina et al., 2003
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dl 

Dorsal

bait

fused to LexA DNA-binding domain

dl 
tamo 

tamo

prey

fused to B42 activation domain

tamo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dl 
RHR domain
sufficient binding region
aa 47-403

coordinates relative to dl-PA

tamo 
Dorsal-binding region
sufficient binding region
aa 385-614

coordinates relative to tamo-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Minakhina et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovarian cDNA expression library).

(Minakhina et al., 2003)
FBrf0158946-5.EMSA.A
Reference
Minakhina et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dl 

Dl

neutral component

GST tag

dl 

Dl

neutral component

GST tag

dl 
dl 
dl 

Dl

neutral component

GST tag

dl 
dl 
tamo 

Tamo

neutral component

His tag

tamo 
tamo 

Tamo

neutral component

His tag

tamo 

Tamo

neutral component

His tag

tamo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dl 
RHR domain
sufficient binding region
aa 47-403

coordinates relative to dl-PA

dl 
NLS
necessary binding region
aa 336-403

coordinates relative to dl-PA

tamo 
Dorsal-binding region
sufficient binding region
aa 385-614

coordinates relative to tamo-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Proteins were mixed with [32]P labeled zen DNA probe. Binding to dl by tamo inferred from inhibition of the zen-dl DNA-protein complex.

(Minakhina et al., 2003)

Positive control.

(Minakhina et al., 2003)

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Minakhina et al., 2003)
FBrf0158946-8.PD.A
Reference
Minakhina et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tamo 

Tamo

bait

GST tag

dl 

Dl

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Minakhina et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)