FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
inc, Grasp65
FlyBase ID
FBig0000107299
Interaction Network
Interactions Browser links
inc network
Grasp65 network
Reported Interactions
FBrf0233997-3a.ITC
Reference
Pirone et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inc 

INC

bait
inc 
Grasp65 

dGRASP

prey
Grasp65 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inc 
BTB domain
sufficient binding region
aa 22-125

coordinates relative to inc-PB

Grasp65 
N-terminal region
sufficient binding region
aa 1-210

coordinates relative to Grasp65-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Pirone et al., 2016)

Kd = 0.7 uM. Analysis of stoichiometry indicates that a Grasp65 monomer binds an inc pentamer.

(Pirone et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)