FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nuf, aPKC
FlyBase ID
FBig0000107520
Interaction Network
Interactions Browser links
nuf network
aPKC network
Reported Interactions
FBrf0233695-4.coIP.MS
Reference
Calero-Cuenca et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait

Tetra tag

nuf 

Nuf

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Calero-Cuenca et al., 2016)
FBrf0233695-5.PD.WB
Reference
Calero-Cuenca et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nuf 

Nuf

bait

GST tag

nuf 
nuf 
nuf 
aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nuf 
N-terminal region
sufficient binding region
aa 1-241

construct "Nt", coordinates relative to nuf-PA

nuf 
residue Ser155
mutation decreasing interaction
aa 155

S155A, coordinates relative to nuf-PA

nuf 
residue Ser155
mutation disrupting interaction
aa 155

S155D, coordinates relative to nuf-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Calero-Cuenca et al., 2016)
FBrf0233695-6.PD.WB
Reference
Calero-Cuenca et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nuf 

Nuf

bait

GST tag

aPKC 

aPKC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Calero-Cuenca et al., 2016)
FBrf0233695-7.coIP.WB
Reference
Calero-Cuenca et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

bait

FLAG tag

aPKC 
nuf 

Nuf

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aPKC 
kinase domain
sufficient binding region
aa 226-606

construct "KD", coordinates relative to aPKC-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic tagged aPKC was isolated from embryos and incubated with purified GST-nuf.

(Calero-Cuenca et al., 2016)

Kinase-dead aPKC did not bind nuf.

(Calero-Cuenca et al., 2016)

Interaction in vitro; bait derived from wild-type embryonic extract; prey produced as a recombinant fusion protein in bacterial system.

(Calero-Cuenca et al., 2016)
FBrf0233695-8.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aPKC 

aPKC

enzyme
aPKC 

aPKC

enzyme
nuf 

Nuf

enzyme target

GST tag, P[32] label

nuf 

Nuf

enzyme target

GST tag, P[32] label

nuf 
nuf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)