FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-184, E2f1
FlyBase ID
FBig0000107615
Interaction Network
Interactions Browser links
mir-184 network
E2f1 network
Reported Interactions
FBrf0211387-1.miR-mRNA.LUC
Reference
Gehrke et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-184 

miR-184

bait
E2f1 

e2f1

prey
E2f1 
E2f1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E2f1 
3-prime UTR
sufficient binding region
E2f1 
mir-184 target site 350
necessary binding region
AUAGGG
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Gehrke et al., 2010)

Luciferase reporter assay.

(Gehrke et al., 2010)
FBrf0211387-4.miR-mRNA.WB
Reference
Gehrke et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-184 

miR-184

bait
E2f1 

e2f1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Overexpression of mir-184 reduces E2f1 protein levels.

(Gehrke et al., 2010)

Source was adult heads of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Gehrke et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)