FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
usp
FlyBase ID
FBig0000107647
Interaction Network
Interactions Browser links
usp network
Reported Interactions
FBrf0183966-2.coIP.WB
Reference
Fang et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
usp 

USP

bait

HA tag

usp 

USP

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fang et al., 2005)
FBrf0206889-3.Y2H
Reference
Bitra and Palli, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
usp 

USP

bait

fused to LexA DNA-binding domain

usp 

USP

prey

fused to B42 activation domain

usp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
usp 
ligand-binding domain
sufficient binding region
aa 172-508

coordinates relative to usp-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bitra and Palli, 2009)

Two-hybrid system: yeast LexA-BD/B42-AD

(Bitra and Palli, 2009)

Positive control.

(Bitra and Palli, 2009)
FBrf0215764-2a.BiFC.FM
Reference
Dutko-Gwóźdź et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
usp 

usp

bait

YFP tag, N-terminal fragment

usp 

usp

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live CHO-K1 or COS-7 cells; proteins produced from transfected constructs.

(Dutko-Gwóźdź et al., 2008)
External Crossreferences and Linkouts ( 0 )
References (3)