FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
His4, Caf1-55
FlyBase ID
FBig0000107919
Interaction Network
Interactions Browser links
His4 network
Caf1-55 network
Reported Interactions
FBrf0191435-7.PD.A
Reference
Furuyama et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Caf1-55 

RbAp48

bait

GST tag

His4 

H4

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Furuyama et al., 2006)
FBrf0200756-1.PD.A
Reference
Tie et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

H4

bait

GST tag

His4 
His4 
Caf1-55 

p55

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His4 
N terminal domain
sufficient binding region
aa 1-85
His4 
N terminal domain
necessary binding region
aa 35-85
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Tie et al., 2007)
FBrf0205275-1.XC
Reference
Song et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Caf1-55 

p55

neutral component
Caf1-55 

p55

neutral component
Caf1-55 

p55

neutral component
His4 

H4

neutral component
His4 
His4 

H4

neutral component
His4 

H4

neutral component
His4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His4 
interaction domain
sufficient binding region
aa 15-41
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion proteins in baculovirus and Sf9 cell system; His4 peptide source unspecified.

(Song et al., 2008)
FBrf0205275-2.SPR
Reference
Song et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

H4

bait
His4 
Caf1-55 

p55

prey
Caf1-55 
Caf1-55 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Caf1-55 
binding loop domain residues 362,365
mutation disrupting interaction
aa 362,365

D362A,D365A

Caf1-55 
N-terminal helix domain residue 35
mutation disrupting interaction
aa 35

L35S

His4 
interaction domain residue 39
mutation disrupting interaction
aa 39

R39A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion proteins in baculovirus and Sf9 cell system; His4 source unspecified.

(Song et al., 2008)
FBrf0213994-1.ITC
Reference
Nowak et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Caf1-55 

Caf1-55

bait
Caf1-55 
Caf1-55 
Caf1-55 
His4 

His4

prey
His4 
His4 
His4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His4 
interaction domain
sufficient binding region
aa 26-45

Kd=35nM.

His4 
interaction domain residue 39
mutation decreasing interaction
aa 39

R39A Kd=115μM.

His4 
interaction domain residues 36,40
mutation decreasing interaction
aa 36,40

R36A,R40A Kd=0.87μM, R36K,R40K Kd=0.11μM.

Caf1-55 
interaction domain residue 358
mutation decreasing interaction
aa 358

Q358A Kd=26μM.

Caf1-55 
interaction domain residues 362,365
mutation decreasing interaction
aa 362,365

D362A,D365A Kd=9.6μM, D362E,D365E Kd=0.44μM, D362N,D365N Kd=1.3μM.

Caf1-55 
interaction domain residues 35,372,373
mutation decreasing interaction
aa 35,372,373

L35S,F372S,I373S Kd=15.4μM.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as recombinant protein in baculovirus and insect cell system; prey peptide synthesized in vitro.

(Nowak et al., 2011)

Binding affinity decreased by competition with Su(z)12 a.a. 1-100 peptide or His3 a.a. 1-28 peptide.

(Nowak et al., 2011)
FBrf0213994-14.PD.MW
Reference
Nowak et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

His4

bait

crosslinked to beads

Caf1-55 

Caf1-55

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptides synthesized in vitro; prey produced as recombinant protein in baculovirus and insect cell system.

(Nowak et al., 2011)

Competition with Su(z)12 abrogates binding.

(Nowak et al., 2011)
FBrf0213994-2.XC
Reference
Nowak et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Caf1-55 

Caf1-55

bait
His4 

His4

prey
His4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His4 
interaction domain
sufficient binding region
aa 26-45
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; recombinant protein produced in baculovirus and insect cell system, peptide synthesized in vitro.

(Nowak et al., 2011)
FBrf0213994-5.PD.MW
Reference
Nowak et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

His4

bait

crosslinked to beads

Caf1-55 

Caf1-55

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptides synthesized in vitro; prey produced as recombinant protein in baculovirus and insect cell system.

(Nowak et al., 2011)

His3, His4 heterodimer used as bait, interaction captured pairwise.

(Nowak et al., 2011)
FBrf0213994-6.CH.MW
Reference
Nowak et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

His4

neutral component
His4 

His4

neutral component
His4 

His4

neutral component
Caf1-55 

Caf1-55

neutral component
Caf1-55 

Caf1-55

neutral component
Caf1-55 

Caf1-55

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

His3, His4, Caf1-55 complex reconstituted in vitro and subjected to gel filtration, interaction captured pairwise.

(Nowak et al., 2011)

Interaction in vitro; peptide synthesized in vitro; recombinant protein produced in baculovirus and insect cell system.

(Nowak et al., 2011)
FBrf0213994-9.CS.MW
Reference
Nowak et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

His4

neutral component
His4 

His4

neutral component
His4 

His4

neutral component
Caf1-55 

Caf1-55

neutral component
Caf1-55 

Caf1-55

neutral component
Caf1-55 

Caf1-55

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

His3, His4, Caf1-55 complex reconstituted in vitro and subjected to analytical ultracentrifugation, interaction captured pairwise.

(Nowak et al., 2011)

Interaction in vitro; peptide synthesized in vitro; recombinant protein produced in baculovirus and insect cell system.

(Nowak et al., 2011)
FBrf0214598-7.PD.MW
Reference
Schmitges et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

H4

bait

GST tag

His4 
Caf1-55 

Nurf55

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His4 
interaction domain
sufficient binding region
aa 1-48
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as recombinant fusion protein in a baculovirus and insect cell system.

(Schmitges et al., 2011)

The His4-Caf1-55 interaction is not detected in the presence of Su(z)12.

(Schmitges et al., 2011)
External Crossreferences and Linkouts ( 0 )
References (5)