FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
PlexA, 14-3-3ε
FlyBase ID
FBig0000108264
Interaction Network
Interactions Browser links
PlexA network
14-3-3ε network
Reported Interactions
FBrf0218615-1.Y2H
Reference
Yang and Terman, 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PlexA 

PlexA

bait

fused to LexA DNA-binding domain

PlexA 
PlexA 
14-3-3ε 

14-3-3ε

prey

fused to B42 activation domain

14-3-3ε 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PlexA 
Cyto2 region
sufficient binding region
aa 1774-1821

coordinates relative to PlexA-PA

PlexA 
14-3-3 binding motif residue S1794
mutation disrupting interaction
aa 1784

S1794A or S1794E, coordinates relative to PlexA-PA

14-3-3ε 
C-terminal region
sufficient binding region
aa 98-261

coordinates relative to 14-3-3ε-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

(Yang and Terman, 2012)

PlexA binds 14-3-3ε but not 14-3-3ζ. 14-3-3ε binds PlexA but not PlexB.

(Yang and Terman, 2012)

Two-hybrid system: yeast LexA-BD/B42-AD

(Yang and Terman, 2012)
FBrf0218615-2.PD.WB
Reference
Yang and Terman, 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

GST tag

PlexA 

PlexA

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic embryonic extract.

(Yang and Terman, 2012)
FBrf0218615-3.coIP.WB
Reference
Yang and Terman, 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PlexA 

PlexA

bait

HA tag

14-3-3ε 

14-3-3ε

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Yang and Terman, 2012)
FBrf0218615-6.coIP.WB
Reference
Yang and Terman, 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PlexA 

PlexA

bait

GST tag

14-3-3ε 

14-3-3ε

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transgenic embryonic extract; prey produced as a recombinant fusion protein in bacterial system.

(Yang and Terman, 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)