FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Fic
FlyBase ID
FBig0000108331
Interaction Network
Interactions Browser links
Fic network
Reported Interactions
FBrf0227106-1.ENZ.A
Reference
Ham et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fic 

dFic

self

GST tag, [32]P label

Fic 

dFic

self

GST tag, [32]P label

Fic 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fic 
residue E247
mutation increasing interaction
aa 247

E247G, coordinates relative to Fic-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A constitutively active form of Fic (E247G) was used for this assay.

(Ham et al., 2014)

Interaction in vitro; enzyme produced as a recombinant fusion protein in bacterial system.

(Ham et al., 2014)

Fic is autoAMPylated.

(Ham et al., 2014)
FBrf0237564-3.CH.MW
Reference
Casey et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fic 

Fic

self

His tag

Fic 

Fic

self

His tag

Fic 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fic 
dimerization domain residue 271
mutation disrupting interaction
aa 271

I271D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Casey et al., 2017)
FBrf0237564-4.CM.MW
Reference
Casey et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fic 

Fic

self

His tag

Fic 

Fic

self

His tag

Fic 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fic 
dimerization domain residue 271
mutation disrupting interaction
aa 271

I271D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Casey et al., 2017)
FBrf0237564-5.ENZ.WB
Reference
Casey et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fic 

Fic

self

His tag

Fic 

Fic

self

His tag

Fic 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fic 
dimerization domain residue 271
mutation decreasing interaction
aa 271

I271D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Casey et al., 2017)

In vitro auto-AMPylation.

(Casey et al., 2017)
External Crossreferences and Linkouts ( 0 )
References (2)