FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108356
Interaction Network
Interactions Browser links
bcd network
cad network
Reported Interactions
FBrf0086389-1.FW.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bcd 

bcd

bait
cad 

cad

prey

[32]P label

cad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cad 
bicoid response element
sufficient binding region
nt 210-553
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected COS cell line; prey produced and labeled by in vitro transcription.

FBrf0086389-2.XL.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cad 

cad

bait

[32]P label

cad 
bcd 

bcd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cad 
bicoid response element
sufficient binding region
nt 210-553
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from transfected COS cell extract.

FBrf0086595-1.XL.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cad 

cad

bait

[32]P label

cad 
bcd 

bcd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cad 
bicoid binding region
sufficient binding region
GCUGGGUGGUAGCGAGCGGGGUGGAGUAGGAGGCGUAGGAGGCGUAGGAGGAGGAGGCGGACAACCGUGCGAUGUGUCCUUCAGUGCUCCCGCUCUCGCCUUGGACUUGGCUUAACCCUUA

"1350-1470" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.

FBrf0086595-2.FW.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bcd 

bcd

bait

GST tag

bcd 
cad 

cad

prey

[32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bcd 
homeodomain
sufficient binding region
aa 216-489

coordinates relative to bcd-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)