Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast LexA-BD/GAL4-AD
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
coordinates relative to MEP-1-PA
coordinates relative to MEP-1-PA
deletion within the context of aa 942-1152.
coordinates correspond to the ttk88 isoform.
coordinates correspond to the ttk69 isoform
The same region in the TTK isoforms ensures the interaction with both regions of MEP-1. The ttk69 interaction domain was confirmed from previous publication.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD